ALOX5AP antibody (Internal Region)

Catalog No. ABIN374740

Product Details anti-ALOX5AP Antibody

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Binding Specificity: Internal Region

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ALOX5AP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-HKVEHESRTQ NGR

Specificity: This antibody recognizes ALOX5AP / FLAP.
Cellular Localization: Nucleus membrane. Endoplasmic reticulum membrane.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001620.2.

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALOX5AP

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Alternative Name: ALOX5AP / FLAP (ALOX5AP Products)

Synonyms: fj65d02 antibody, zgc:63982 antibody, wu:fj65d02 antibody, flap antibody, al5ap antibody, Alox5ap antibody, ALOX5AP antibody, Flap antibody, FLAP antibody, arachidonate 5-lipoxygenase-activating protein antibody, arachidonate 5-lipoxygenase activating protein antibody, alox5ap antibody, ALOX5AP antibody, Alox5ap antibody

Background: Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367], also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.Synonyms: Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein

Gene ID: 241, 9606

UniProt: P20292