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NCF4 antibody (Internal Region)

NCF4 Reactivity: Human, Dog WB, IHC (p), EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN374770
  • Target See all NCF4 Antibodies
    NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
    Binding Specificity
    • 19
    • 15
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 81
    • 25
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Dog
    Host
    • 76
    • 5
    • 1
    Goat
    Clonality
    • 78
    • 4
    Polyclonal
    Conjugate
    • 37
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This NCF4 antibody is un-conjugated
    Application
    • 41
    • 26
    • 26
    • 20
    • 16
    • 12
    • 7
    • 4
    • 4
    • 3
    • 3
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody reacts to NCF4 / P40PHOX.
    Cross-Reactivity (Details)
    Species reactivity (expected):Canine.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Immunogen
    Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence
    Top Product
    Discover our top product NCF4 Primary Antibody
  • Application Notes
    Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in lysates of cell line Daudi (calculated MW of39.8 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). Intransfected HEK293 transiently expressing P40PHOX a band of approx.48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Tonsil shows staining of thecytoplasm in some cells. Recommended concentration: 2 - 4 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
    Alternative Name
    NCF-4 (NCF4 Products)
    Synonyms
    p40phox antibody, AI451400 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody, neutrophil cytosolic factor 4 antibody, ncf4 antibody, Ncf4 antibody, NCF4 antibody
    Background
    NCF4 is a cytosolic oxidase associated protein. It is a component of the nicotinamide adenine dinucleotide phosphate oxidase, which mediates down regulation of NADPH oxidase through interactions with its SH3 domain. NCF4 is associated with p67 phox but is absent in patients with chronic granulomatous disease who lack p67 phox.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox
    Gene ID
    4689
    NCBI Accession
    NP_000622
    UniProt
    Q15080
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