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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody

Details for Product No. ABIN374770, Supplier: Login to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
Epitope
Internal Region
17
12
12
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
111
47
37
6
5
3
3
1
1
Host
Goat
83
19
12
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
71
42
32
22
18
6
3
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence
Specificity This antibody reacts to NCF4 / P40PHOX.
Cross-Reactivity (Details) Species reactivity (expected):Canine.
Species reactivity (tested):Human.
Purification Affinity chromatography
Alternative Name NCF-4 (NCF4 Antibody Abstract)
Background NCF4 is a cytosolic oxidase associated protein. It is a component of the nicotinamide adenine dinucleotide phosphate oxidase, which mediates down regulation of NADPH oxidase through interactions with its SH3 domain. NCF4 is associated with p67 phox but is absent in patients with chronic granulomatous disease who lack p67 phox.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox
Gene ID 4689
NCBI Accession NP_000622
UniProt Q15080
Pathways
Application Notes Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in lysates of cell line Daudi (calculated MW of39.8 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). Intransfected HEK293 transiently expressing P40PHOX a band of approx.48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Tonsil shows staining of thecytoplasm in some cells. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (ABIN374770) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (ABIN374770) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (Image 2)
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (ABIN374770) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody (Image 3)
Background publications Rioux, Xavier, Taylor et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. ..." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).