PLIN1 antibody (C-Term)

Catalog No. ABIN374794

Product Details anti-PLIN1 Antibody

Target: PLIN1 (Perilipin 1 (PLIN1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PLIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: C-EPILGRAQYS QLRKK

Specificity: This antibody detects Perilipin-1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the C-terminus of Human PLIN (NP_002657.2) Genename: PLIN

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx 55-60 kDa band observed in Human Adipose lysates(calculated MW of 56.0 kDa according to NP_002657.2). Immunofluorescence: 1 μg/mL. Small lipid droplets preferentially stained in the periphery ofdifferentiated 3T3-L1 adipocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for PLIN1

Target: PLIN1 (Perilipin 1 (PLIN1))

Alternative Name: Perilipin-1 (PLIN1 Products)

Synonyms: FPLD4 antibody, PERI antibody, PLIN antibody, PERIA antibody, Plin antibody, 6030432J05Rik antibody, Peri antibody, peri antibody, plin antibody, perilipin antibody, LOC692833 antibody, perilipin 1 antibody, perilipin antibody, perilipin 1 L homeolog antibody, PLIN1 antibody, Plin1 antibody, plin1 antibody, LOC692833 antibody, plin1.L antibody

Background: Synonyms: Lipid Droplet-Associated Protein, PERI, PLIN, PLIN-1, PLIN1, Perilipin

Gene ID: 5346, 9606

UniProt: O60240

Pathways: Lipid Metabolism