AOC3 antibody (C-Term, Internal Region)

Catalog No. ABIN374864

Product Details anti-AOC3 Antibody

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Binding Specificity: C-Term, Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This AOC3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DEDPSFYSAD SIY

Specificity: This antibody detects internal AOC3 / VAP1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_003725.1 Genename: AOC3

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Preliminary experiments gave an approx 55 kDa band in Human Adipose andLung lysates after 0.01 μg/mL antibody staining. Please note that currently we cannot findan explanation in the literature for the band we observe given the calculated size of84.5 kDa according to NP_003725.1. The 55 kDa band was successfully blocked byincubation with the immunizing peptide. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Liver shows membranousstaining in sinusoid lining cells surrounding hepatocytes.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for AOC3

Target: AOC3 (Amine Oxidase, Copper Containing 3 (Vascular Adhesion Protein 1) (AOC3))

Alternative Name: AOC3 / VAP1 (AOC3 Products)

Synonyms: HPAO antibody, SSAO antibody, VAP-1 antibody, VAP1 antibody, aoc2 antibody, BOLAO antibody, amine oxidase, copper containing 3 antibody, amine oxidase, copper containing 3 L homeolog antibody, AOC3 antibody, aoc3.L antibody, Aoc3 antibody

Background: VAP1, upregulated in response to inflammation, is a cell adhesion protein that participates in lymphocyte recirculation, by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells, in an L selectin independent fashion. VAP1 also displays a monoamine oxidase activity, catalyzing the deamination of methylamine to produce formaldehyde. Upregulation of VAP1 has been reported in Alzheimer's disease, where increased levels of formaldehyde are responsible for b amyloid cross linkage and plaque formation, significantly contributing to the pathogenesis of this disease.Synonyms: Copper amine oxidase, HPAO, Membrane primary amine oxidase, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1

Gene ID: 8639, 9606

UniProt: Q16853

Pathways: Feeding Behaviour