Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: 0.01-0.03 μg/mL. Approx 75 kDa band observed in lysates of cell line Daudi(calculated MW of 67.5 kDa according to NP_003191.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play major roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'. Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.Synonyms: ITF-1, ITF1, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, TCF-3, Transcription factor 3, Transcription factor E2-alpha, Transcription factor ITF-1