TBC1D4 antibody (Internal Region)

Catalog No. ABIN374867

Product Details anti-TBC1D4 Antibody

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Binding Specificity: Internal Region

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DDPEKIEERK KSK

Specificity: This antibody detects internal TBC1D4 / AS160.

Cross-Reactivity (Details): Species reactivity (expected):DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_055647.2 Genename: TBC1D4

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 128000. Western Blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4(calculated MW of 140 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4 / AS160 (TBC1D4 Products)

Synonyms: AS160 antibody, 5930406J04Rik antibody, A930035N22 antibody, AV295684 antibody, As160 antibody, RGD1561609 antibody, TBC1 domain family member 4 antibody, TBC1 domain family, member 4 antibody, TBC1D4 antibody, Tbc1d4 antibody

Background: TBC1D4 is a Rab GTPase activating protein (GAP) for Rab family protein(s) (referenced from swissprot).Synonyms: Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4

Gene ID: 9882, 9606

UniProt: O60343