GCNT3 antibody (Internal Region)

Catalog No. ABIN374918

Product Details anti-GCNT3 Antibody

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Binding Specificity: AA 410-422, Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GCNT3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-NKFDPKVDDN ALQ

Specificity: This antibody detects C2GnT-M (410-422) .

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region (near C terminus) of the protein sequence according to NP_004742.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GCNT3

Target: GCNT3 (Glucosaminyl (N-Acetyl) Transferase 3, Mucin Type (GCNT3))

Alternative Name: GCNT3 (GCNT3 Products)

Synonyms: C2/4GnT antibody, C24GNT antibody, C2GNT2 antibody, C2GNTM antibody, GNTM antibody, 2010013H22Rik antibody, 2210021I22Rik antibody, 2210401J11Rik antibody, beta-16-N-acetylglucosaminyltransferase antibody, dI/C2/C4GnT antibody, c2/4gnt antibody, c24gnt antibody, c2gnt2 antibody, c2gntm antibody, gntm antibody, glucosaminyl (N-acetyl) transferase 3, mucin type antibody, glucosaminyl (N-acetyl) transferase 3, mucin type L homeolog antibody, GCNT3 antibody, Gcnt3 antibody, gcnt3.L antibody

Background: GCNT3 is a glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. GCNT3 mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. GCNT3 has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. GCNT3 belongs to the glycosyltransferase 14 family.Synonyms: 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase, 6-N-acetylglucosaminyltransferase 3, Beta-1, C2/4GnT, C2GnT-mucin type, Core 2/core 4 beta-1

Gene ID: 9245

NCBI Accession: NP_004742

UniProt: O95395

Pathways: Production of Molecular Mediator of Immune Response