Lactate Dehydrogenase C antibody (Internal Region)

Catalog No. ABIN374990

Product Details anti-Lactate Dehydrogenase C Antibody

Target: Lactate Dehydrogenase C (LDHC)

Binding Specificity: AA 221-233, Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-DSDKEHWKNV HKQ

Specificity: This antibody recognizes LDHC (221-233).

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002292.1 and NP_059144.1. Genename: LDHC

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.03 - 0.1 μg/mL. Immunohistochemistry: Strong signal in seminiferous tubules of Mouse Testis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lactate Dehydrogenase C

Target: Lactate Dehydrogenase C (LDHC)

Alternative Name: LDHC (LDHC Products)

Synonyms: CT32 antibody, LDH3 antibody, LDHX antibody, LDH-C4 antibody, Ldh-3 antibody, Ldh-x antibody, Ldh3 antibody, Ldhc4 antibody, LDH-C antibody, LDH-X antibody, lactate dehydrogenase C antibody, L-lactate dehydrogenase C chain antibody, LDHC antibody, Ldhc antibody, ldhc antibody, LOC100713414 antibody, LOC100343171 antibody

Background: Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes Powered by Epitomics Technology.Synonyms: CT32, Cancer/testis antigen 32, EC=1.1.1.27, L-lactate dehydrogenase C chain, LDH testis subunit, LDH-C, LDH-X, LDHC

Gene ID: 16833

NCBI Accession: NP_002292

UniProt: P07864

Pathways: Ribonucleoside Biosynthetic Process, Warburg Effect