ERP29 antibody (C-Term)

Catalog No. ABIN375036

Product Details anti-ERP29 Antibody

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ERP29 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to ERP29.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EKNKMSDGKKEELQ, from the C-Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ERP29

Target: ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))

Alternative Name: ERP29 (ERP29 Products)

Synonyms: C12orf8 antibody, ERp28 antibody, ERp31 antibody, PDI-DB antibody, PDIA9 antibody, 1200015M03Rik antibody, 2810446M09Rik antibody, AW209030 antibody, Erp28 antibody, Erp31 antibody, PDI-Db antibody, endoplasmic reticulum protein 29 antibody, ERP29 antibody, Erp29 antibody

Background: Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI's thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.Synonyms: C12orf8, ERp28, ERp31, Endoplasmic reticulum protein ERp29

Gene ID: 10961

NCBI Accession: NP_006808

UniProt: P30040