BHMT antibody (C-Term)

Catalog No. ABIN375080

Product Details anti-BHMT Antibody

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-EQQLKELFEK QK

Specificity: This antibody recognizes BHMT.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence Genename: BHMT

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT (BHMT Products)

Synonyms: BHMT1 antibody, hm:zehn2153 antibody, wu:fb53h01 antibody, wu:fb63c08 antibody, wu:fj64d01 antibody, zgc:123027 antibody, betaine--homocysteine S-methyltransferase antibody, betaine--homocysteine S-methyltransferase L homeolog antibody, betaine-homocysteine methyltransferase antibody, betaine-homocysteine S-methyltransferase antibody, BHMT antibody, bhmt.L antibody, Bhmt antibody, bhmt antibody

Background: BHMT (Betaine--homocysteine S-methyltransferase 1) is involved in the regulation of homocysteine metabolism. It is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. It is expressed exclusively in liver and kidney and displays differential expression in a model of liver cirrhosis.Synonyms: Betaine-homocysteine S-methyltransferase 1

Molecular Weight: 44.9kDa (Human NP_001704.1), 45.0kDa (Mouse NP_057877.1 and Rat NP_110477.1).

Gene ID: 635, 9606

UniProt: C6KNH7

Pathways: Methionine Biosynthetic Process