GABRA4 antibody (Internal Region)

Catalog No. ABIN375085

Product Details anti-GABRA4 Antibody

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Binding Specificity: Internal Region

Reactivity: Human, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This GABRA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to GABRA4.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EKAKRKTSKPPQE, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx.70 kDa band observed in Human Brain (Cerebellum and FrontalCortex) lysates (calculated MW of 61.0 kDa according to NP_000800.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Human Cortex shows patchy staining near orat the neuronal cell membrane. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GABRA4

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Alternative Name: GABRA4 (GABRA4 Products)

Synonyms: GABRA4 antibody, Gabra-4 antibody, gamma-aminobutyric acid type A receptor alpha4 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4 antibody, GABRA4 antibody, Gabra4 antibody

Background: GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.Synonyms: GABAA Receptor alpha-4, GABRA-4, Gamma-Aminobutyric Acid Receptor subunit Alpha-4

Gene ID: 2557

NCBI Accession: NP_000800

UniProt: P48169

Pathways: Synaptic Membrane