SULF2 antibody (Internal Region)

Catalog No. ABIN375090

Product Details anti-SULF2 Antibody

Target: SULF2 (Sulfatase 2 (SULF2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SULF2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is expected to recognize both reported isoforms (NP_061325.1 and NP_940998.1).

Purification: Affinity Chromatography

Immunogen: Peptide with sequence C-DGRVYHVGLGDAAQPR, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SULF2

Target: SULF2 (Sulfatase 2 (SULF2))

Alternative Name: SULF2 (SULF2 Products)

Synonyms: Sulf1 antibody, zgc:55612 antibody, SULF2 antibody, si:dkeyp-84g9.1 antibody, wu:fb48e04 antibody, HSULF-2 antibody, xtsulf2 antibody, 2010004N24Rik antibody, AU020235 antibody, MSulf-2 antibody, mKIAA1247 antibody, sulfatase 2a antibody, sulfatase 2 antibody, extracellular sulfatase Sulf-2 antibody, sulfatase 2b antibody, sulfatase 2 S homeolog antibody, sulf2a antibody, SULF2 antibody, LOC100540921 antibody, sulf2 antibody, sulf2b antibody, sulf2.S antibody, Sulf2 antibody

Background: Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Mammalian Sulfs are endoproteolytically processed and secreted into the extracellular space of transfected cells, where they exhibit both arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity against intact heparin. Though the molecular weight of full length human Sulf2 is 100455 Da (870 amino acids), the endogenous Sulf2 is found by western blot to be present at molecular weights of 24.4, 33.7 and 51-60 kDa. Higher molecular weight species may be found depending on tissue and/or cell type. Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma. Tumors formed by cells expressing Sulf demonstrate enhanced extracellular matrix deposition. The 6-O-sulfation of heparan sulfate of myeloma tumor cells may be a critical factor in determiningand regulating the in vivo growth of this cancer.Synonyms: Extracellular sulfatase Sulf-2, KIAA1247

Gene ID: 55959, 9606

UniProt: Q8IWU5