MED15 antibody (both Isoforms, Internal Region)

Catalog No. ABIN375100

Product Details anti-MED15 Antibody

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Binding Specificity: both Isoforms, Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to PCQAP / MED15.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PPQSQTQPLVSQAQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15 (MED15 Products)

Synonyms: Arc105 antibody, BcDNA:GH03922 antibody, CG4184 antibody, Dmel\\CG4184 antibody, Med15 antibody, Med9/ARC105 antibody, dARC105 antibody, med15 antibody, PCQAP antibody, MED15 antibody, DKFZp459O0325 antibody, ARC105 antibody, CAG7A antibody, CTG7A antibody, TIG-1 antibody, TIG1 antibody, TNRC7 antibody, A230074L19Rik antibody, AW536074 antibody, Pcqap antibody, arc105 antibody, fi85b10 antibody, pcqap antibody, wu:fd19g01 antibody, wu:fi19d01 antibody, wu:fi85b10 antibody, zgc:55839 antibody, Mediator complex subunit 15 antibody, mediator complex subunit 15 antibody, mediator of RNA polymerase II transcription subunit 15 antibody, mediator complex subunit 15 L homeolog antibody, MED15 antibody, LOC100195848 antibody, Med15 antibody, med15 antibody, med15.L antibody

Background: PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.Synonyms: ARC105, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, CTG7A, Mediator complex subunit 1, Mediator of RNA polymerase II transcription subunit 15, PCQAP, Positive cofactor 2 glutamine/Q-rich-associated protein, TIG1, TNRC7, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein

Gene ID: 51586, 9606

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha