Patched 1 antibody (Internal Region)

Catalog No. ABIN375146

Product Details anti-Patched 1 Antibody

Target: Patched 1 (PTCH1)

Binding Specificity: Internal Region

Reactivity: Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Sequence: C-QTGSKKEPFN YSQ

Specificity: This antibody recognizes internal PTCH1.

Cross-Reactivity (Details): Species reactivity (tested):Zebrafish.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_571063.1

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Immunofluorescence studies of Zebrafish tumors: 1-3 μg/mL (Ju et al, Mol Cancer.2009 Jun25,8: 40. PMID: 19555497).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1 (PTCH1 Products)

Synonyms: BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody

Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.Synonyms: PTC, PTC1, PTCH, Patched 1, Protein patched homolog 1

Gene ID: 30181, 7955

UniProt: Q98864

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation