IFT57 antibody (Internal Region)

Catalog No. ABIN375157

Product Details anti-IFT57 Antibody

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT57 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to HIPPI.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EQPQEYDDPNATISN, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: HIP1-Interacting Protein (IFT57 Products)

Synonyms: Esrrbl1 antibody, 4833420A15Rik antibody, Hippi antibody, MHS4R2 antibody, ESRRBL1 antibody, HIPPI antibody, intraflagellar transport 57 antibody, intraflagellar transport 57 S homeolog antibody, Ift57 antibody, IFT57 antibody, ift57.S antibody

Background: HIPPI was identified as a protein that interacts with HIP1 (Huntingtin-interacting protein 1). HIPPI mRNA is expressed in the brain. It is present in all regions of the brain, and to a lesser extent in spinal cord. It is also expressed abundantly in heart, kidney, and lung, with weaker expression in all other tissues tested. HIPPI is expressed in the Golgi apparatus of neurons where it partly colocalizes with HIP1. HIPPI forms a heterodimer with HIP1. The availability of free HIP1 is modulated by polyglutamine length within huntingtin, with disease-associated polyglutamine expansion favoring the formation of proapoptotic HIPPI-HIP1 heterodimers. These heterodimers can recruit procaspase-8 into a complex of HIPPI, HIP1, and procaspase-8 and launch apoptosis through components of the extrinsic cell death pathway. This novel non-receptor-mediated pathway for activating caspase-8 might contribute to neuronal death in Huntington disease.Synonyms: DERP8, Dermal papilla-derived protein 8, ESRRBL1, Estrogen-related receptor beta-like protein 1, HIPPI, IFT57, Intraflagellar transport protein 57 homolog, MHS4R2

Gene ID: 55081, 9606

UniProt: Q9NWB7

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity