CHRNA4 antibody (Internal Region)

Catalog No. ABIN375184

Product Details anti-CHRNA4 Antibody

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Binding Specificity: AA 29-43, Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-HVETRAHAEE RLLKK

Specificity: This antibody recognizes CHRNA4 (aa29-43). This antibody is expected Not to cross-react with the similar alpha 2 subunit.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Bovine, Canine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the internal region of Human CHRNA4 (NP_000735.1) Genename: CHRNA4

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: Neuronal Acetylcholine Receptor Subunit alpha-4 (CHRNA4 Products)

Synonyms: NACHRA4 antibody, BFNC antibody, EBN antibody, EBN1 antibody, NACHR antibody, NACRA4 antibody, NARAC antibody, Acra-4 antibody, Acra4 antibody, ENFL1 antibody, neuronal acetylcholine receptor subunit alpha-4 antibody, neuronal acetylcholine receptor subunit alpha-4-like antibody, cholinergic receptor nicotinic alpha 4 subunit antibody, cholinergic receptor, nicotinic, alpha 4b antibody, cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, CpipJ_CPIJ002434 antibody, LOC100368057 antibody, CHRNA4 antibody, chrna4b antibody, Chrna4 antibody

Background: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. After binding acetylcholine, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in nACHR4 are the cause of autosomal dominant nocturnal frontal lobe epilepsy type 1 (ENFL1) also symbolized ADNFLE. ENFL1 is a disease characterized by clusters of motor seizures during sleep. Polymorphisms in this gene that provide protection against nicotine addiction have been described.Synonyms: CHRNA4, NACRA4, Nicotinic acetylcholine receptor subunit alpha 4

Molecular Weight: 70.0 kDa (Human NP_000735.1) and 70.2 kDa (Rat NP_077330.1)

Gene ID: 25590

NCBI Accession: NP_077330

UniProt: P09483

Pathways: Synaptic Membrane