COX IV antibody (C-Term, Isoform 1, Isoform 2)

Catalog No. ABIN375186

Product Details anti-COX IV Antibody

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Binding Specificity: C-Term, Isoform 1, Isoform 2

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QGLASKWDYE KNE

Specificity: This antibody recognizes both COX4l1 and COX4l2.

Cross-Reactivity (Details): Species reactivity (tested):Human.
Also reacts to: isoform 2

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_001852.1.

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: Use at 0.01-0.03 μg/mL. Approx 18 kDa band observed in human skeletal muscle lysates(Calculated MW of 19.8 kDa according to NP_001852.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris-saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX IV (COX4I1 Products)

Synonyms: COX4 antibody, COX4-1 antibody, COXIV antibody, mg:bb02d03 antibody, zgc:110058 antibody, GB20012 antibody, AL024441 antibody, Cox4 antibody, Cox4a antibody, cox4 antibody, cox4i1 antibody, coxiv antibody, BcDNA:AT07685 antibody, CG10396 antibody, COX IV antibody, CX41 antibody, CX42 antibody, Dmel\CG10396 antibody, IV antibody, cytochrome c oxidase subunit 4I1 antibody, cytochrome c oxidase subunit IV isoform 1 antibody, cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV antibody, cytochrome c oxidase subunit 4i1 antibody, cytochrome c oxidase subunit IV isoform 1 S homeolog antibody, Cytochrome c oxidase subunit 4-like antibody, COX4I1 antibody, cox4i1 antibody, LOC412396 antibody, Cox4I1 antibody, LOC780848 antibody, BMEII0322 antibody, RSal33209_1582 antibody, SJAG_00976 antibody, HMPREF0733_11926 antibody, Cox4i1 antibody, cox4i1.S antibody, COX4L antibody

Background: Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).Synonyms: COX IV-1, COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, mitochondrial Cytochrome c oxidase subunit 4 isoform 1

Gene ID: 1327

NCBI Accession: NP_001852

UniProt: P13073

Pathways: Proton Transport