ITCH antibody (Internal Region)

Catalog No. ABIN375211

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to ITCH.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH / AIF4 (ITCH Products)

Synonyms: AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody

Background: ITCH interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells (referenced from Swiss-Prot).Synonyms: Atrophin-1-interacting protein 4, E3 ubiquitin-protein ligase Itchy homolog, NAPP1, NFE2-associated polypeptide 1

Gene ID: 83737

NCBI Accession: NP_113671

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events