MARK2 antibody (Internal Region)

Catalog No. ABIN375213

Product Details anti-MARK2 Antibody

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MARK2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibdoy reacts to MARK2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-QNGKDSTAPQR, from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/16000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MARK2

Target: MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))

Alternative Name: MARK2 (MARK2 Products)

Synonyms: EMK-1 antibody, EMK1 antibody, PAR-1 antibody, Par-1b antibody, Par1b antibody, AU024026 antibody, Emk antibody, Par-1 antibody, mKIAA4207 antibody, microtubule affinity regulating kinase 2 antibody, MAP/microtubule affinity regulating kinase 2 antibody, MARK2 antibody, Mark2 antibody

Background: MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein. Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.Synonyms: 2, ELKL motif kinase, EMK1, MAP/microtubule affinity-regulating kinase, MARK2, PAR1 homolog, Serine/threonine-protein kinase

Gene ID: 2011

NCBI Accession: NP_001034558

UniProt: Q7KZI7

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection