CD8 alpha antibody (Cy5)

Catalog No. ABIN376062

Product Details anti-CD8 alpha Antibody (Cy5)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to Cy5

Application: Flow Cytometry (FACS)

Specificity: Chicken/Turkey/Guinea Fowl/Pigeon CD8α, Mr 34 kDa

Characteristics: Mouse Anti-Chicken CD8a-CY5

Purification: Purified

Immunogen: Chicken thymocytes and Ig-negative blood leukocytes

Clone: CT-8

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and CY5 conjugates 0.2 g/106 cells APC, SPRD, AF647, and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.1 mg in 0.2 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (Cy5) (ABIN376062)

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a (CD8A Products)

Background: In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two α chains, and (ii) a heterodimer of an α chain and a β chain. While the vast majority of CD8 + cells in the thymus, spleen, and blood of adult chickens express both CD8 α - and CD8 β -chains, a relatively large proportion of the CD8 + TCR- γδ cells in the spleens of embryos and young chicks express only the α -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8 + T cell populations present in mice are conserved, but there is a population of TCR- γδ CD8 αβ cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80 % of thymocytes, 15 % of blood mononuclear cells and 50 % of spleen cells, but < 1 % of cells in the bursa and bone marrow.

Pathways: TCR Signaling