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Goat anti-Mouse (Murine) IgG Antibody (HRP)

Details for Product No. ABIN376241, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
2644
2621
2280
1629
1374
735
603
422
361
333
329
321
308
258
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Goat
6518
4478
1762
774
636
365
87
51
50
9
8
5
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
2102
1798
1649
1306
717
630
522
296
294
284
274
267
216
187
178
117
101
79
69
55
54
40
35
25
23
22
19
19
19
19
18
15
15
15
15
15
15
15
14
13
12
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with mouse IgG 1, IgG 2a, IgG 2b and IgG 3 as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Purification Affinity chromatography on mouse IgG 3 covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Gao, Yallampalli, Yallampalli: "Gestational protein restriction increases angiotensin II production in rat lung." in: Biology of reproduction, Vol. 88, Issue 3, pp. 64, 2013 (PubMed).

Kamphuis, Shafique, Meijerhof, Stegmann, Wilschut, de Haan: "Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats." in: Vaccine, 2013 (PubMed). Further details: Plates were incubated with a 1:5000 dilution of horseradish-peroxidase-coupled goat anti-mouse IgG, or IgA which bind to both mouse and cotton rat IgG and IgA, respectively

Chen, Lin, Wang: "Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach." in: Clinical biochemistry, 2011 (PubMed).

Kim, Welsch, Yi, Lemon: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).