Goat anti-Mouse (Murine) IgG Antibody (HRP)

Details for Product No. ABIN376241, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
2816
2785
2375
1734
1463
781
607
425
365
338
331
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Goat
6774
4670
1876
826
703
396
88
54
52
21
9
8
5
5
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2205
1871
1720
1369
765
679
519
297
297
284
274
268
217
188
178
124
103
79
69
60
54
40
35
32
29
22
22
20
19
19
19
19
16
16
15
15
15
15
15
14
12
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with mouse IgG 1, IgG 2a, IgG 2b and IgG 3 as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Purification Affinity chromatography on mouse IgG 3 covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Gao, Yallampalli, Yallampalli: "Gestational protein restriction increases angiotensin II production in rat lung." in: Biology of reproduction, Vol. 88, Issue 3, pp. 64, 2013 (PubMed).

Kamphuis, Shafique, Meijerhof, Stegmann, Wilschut, de Haan: "Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats." in: Vaccine, 2013 (PubMed). Further details: Plates were incubated with a 1:5000 dilution of horseradish-peroxidase-coupled goat anti-mouse IgG, or IgA which bind to both mouse and cotton rat IgG and IgA, respectively

Chen, Lin, Wang: "Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach." in: Clinical biochemistry, 2011 (PubMed).

Kim, Welsch, Yi, Lemon: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).