Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

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Antigen
Epitope
Heavy & Light Chain
4683
2573
1882
1039
1015
825
305
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Rabbit
2780
2760
2346
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Goat
6749
4661
1834
812
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2182
1862
1718
1362
765
675
521
297
295
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification Affinity chromatography on pooled human IgA covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Kim, Welsch, Yi, Lemon: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).