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Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN376289, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4507
1737
1715
896
817
633
618
279
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
2693
2670
2307
1710
1413
743
613
423
351
337
336
332
312
262
165
149
123
112
40
27
21
21
12
8
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Host
Goat
6684
4615
1763
765
653
367
93
51
50
8
8
5
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
2126
1816
1691
1299
720
633
528
313
303
303
301
283
262
222
217
117
81
79
69
54
49
39
36
25
23
21
19
18
15
15
15
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification Affinity chromatography on pooled human IgA covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Kim, Welsch, Yi, Lemon: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).