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Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN376289, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4517
1867
1732
962
643
277
203
56
35
34
31
8
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
2468
2360
2054
1545
1279
628
581
401
328
315
315
309
293
224
164
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Goat
6099
4268
1527
605
545
298
85
49
43
8
8
4
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
1915
1743
1552
1135
605
529
523
265
198
181
172
161
156
155
127
79
79
69
43
41
37
32
32
31
23
20
19
19
17
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification Affinity chromatography on pooled human IgA covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Kim, Welsch, Yi et al.: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).