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TCRab/Vb2 antibody (SPRD)

Reactivity: Chicken FACS Host: Mouse Monoclonal TCR-3 SPRD
Catalog No. ABIN376730
  • Target
    TCRab/Vb2
    Reactivity
    Chicken
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    SPRD
    Application
    Flow Cytometry (FACS)
    Brand
    Spectral Red ™
    Specificity
    Chicken/Peacock/Guinea Fowl TCRαβ/Vβ2, Mr 48 & 40 kDa
    Characteristics
    Mouse Anti-Chicken TCRab/Vb2-SPRD
    Purification
    Purified
    Immunogen
    CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
    Clone
    TCR-3
    Isotype
    IgG1
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Depletion - Reported in literature
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.5 g/106 cells SPRD conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Comment

    In ovo suppression of the development of TCRab-Vb2 cell

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    TCRab/Vb2
    Background
    In the chicken, two distinct subpopulations of αβ T cells appear in the thymus subsequent to the appearance of γδ T cells. These subpopulations, originally denoted as TCR2 and TCR3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct V β families, V β 1 and V β 2, respectively. The TCR3 MAb reacts with approximately 9 % of thymocytes, 15-25 % of blood mononuclear cells and 13 % of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3 + thymocytes include CD4 + CD8 -, CD4 - CD8 +, CD4 + CD8 + and CD4 - CD8 - subpopulations. The TCR3 + thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4 + CD8 +. Cells in the other subset express TCR3/CD3 in higher density and are either CD4 + CD8 - or CD4 - CD8 +, corresponding to the more mature medullary subset of thymocytes. The TCR3 + cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are “single positive, with CD4 + CD8 - cells being four times more frequent that the CD4 - CD8 + cells (ca. 80 % CD4 + vs ca. 20 % CD8 + ).
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