His Tag antibody

Details for Product No. ABIN387699, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
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14
3
2
Host
Mouse
434
184
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
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4
4
4
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3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
554
385
166
106
98
77
69
26
13
11
9
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4
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2
1
1
1
1
1
1
1
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1
Options
Supplier
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Supplier Product No.
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Brand THE™
Immunogen A synthetic peptide HHHHHH coupled - KLH
Sequence HHHHHH
Clone 6G2A9
Isotype IgG
Specificity THETMHis Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Characteristics Anti-His mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. Anti-His mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. Anti-His mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Purification Protein A affinity column
Target Type Tag
Background Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at successfully isolated an antibody against His-tag. Anti-His mAb (subtype IgG1) has very high affinity. Tests performed at show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-0.2 µg/mL
Immunoprecipitation (IP): 1 µg/mL
Immunofluorescent staining: 1 µg/mL
Flow cytometry: 1 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Supplier Images
 image for anti-His Tag antibody (ABIN387699) Lane 1: 6x His Protein Ladder. Lane 2: N-terminal His-fusion proteinLane 3: C-termina...
 image for anti-His Tag antibody (ABIN387699) Lane 1-3: Multiple Tag Cell Lysate (ABIN1536505) Lane 4: Negative controlPrimary Anti...
 image for anti-His Tag antibody (ABIN387699) A. Antibodies-Online (ABIN387699) B. Company Q#1C. Company Q#2Note: Whole cell lysate...
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Stenner, Liewen, Göttig, Henschler, Markuly, Kleber, Faust, Mischo, Bauer, Zweifel, Knuth, Renner, Wadle: "RP1 is a phosphorylation target of CK2 and is involved in cell adhesion." in: PLoS ONE, Vol. 8, Issue 7, pp. e67595, 2013 (PubMed).

Son, Shen, Margueron, Reinberg: "Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin." in: Genes & development, Vol. 27, Issue 24, pp. 2663-77, 2013 (PubMed).

Guler, Liu, Vaithiyalingam, Arnett, Kremmer, Chazin, Fanning: "Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress." in: The Journal of biological chemistry, Vol. 287, Issue 9, pp. 6469-81, 2012 (PubMed).

Rubio-Infante, Govea-Alonso, Alpuche-Solís, García-Hernández, Soria-Guerra, Paz-Maldonado, Ilhuicatzi-Alvarado, Varona-Santos, Verdn-Terán, Korban, Moreno-Fierros, Rosales-Mendoza: "A chloroplast-derived C4V3 polypeptide from the human immunodeficiency virus (HIV) is orally immunogenic in mice." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 337-49, 2012 (PubMed).

Amparyup, Sutthangkul, Charoensapsri, Tassanakajon: "Pattern recognition protein binds to lipopolysaccharide and ?-1,3-glucan and activates shrimp prophenoloxidase system." in: The Journal of biological chemistry, Vol. 287, Issue 13, pp. 10060-9, 2012 (PubMed).

Horstman, Darwin: "Phage shock proteins B and C prevent lethal cytoplasmic membrane permeability in Yersinia enterocolitica." in: Molecular microbiology, Vol. 85, Issue 3, pp. 445-60, 2012 (PubMed).

Qian, Zhu, Li, Belevych, Chen, Zhao, Herness, Quan: "Interleukin-1R3 mediates interleukin-1-induced potassium current increase through fast activation of Akt kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 30, pp. 12189-94, 2012 (PubMed).

Wang, Zhao, Sun, Liu, Weng: "Enhancing catalytic activity of a hybrid xylanase through single substitution of Leu to Pro near the active site." in: World journal of microbiology & biotechnology, Vol. 28, Issue 3, pp. 929-35, 2012 (PubMed).

Frank, McDonald, Karata, Huston, Woodgate: "A strategy for the expression of recombinant proteins traditionally hard to purify." in: Analytical biochemistry, Vol. 429, Issue 2, pp. 132-9, 2012 (PubMed).

Reitter, Mills: "Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif." in: Journal of bacteriology, Vol. 193, Issue 4, pp. 994-7, 2011 (PubMed).

Chatfield, Mulhern, Burnside, Cianciotto: "Legionella pneumophila LbtU acts as a novel, TonB-independent receptor for the legiobactin siderophore." in: Journal of bacteriology, Vol. 193, Issue 7, pp. 1563-75, 2011 (PubMed).

De la Mota-Peynado, Chernoff, Beeser: "Identification of the atypical MAPK Erk3 as a novel substrate for p21-activated kinase (Pak) activity." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13603-11, 2011 (PubMed).

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