DYKDDDDK Tag antibody

Details for Product No. ABIN387700, Supplier: Log in to see
Antigen
Reactivity
Tag
233
121
65
18
18
16
9
7
4
3
2
1
1
1
Host
Mouse
194
136
34
34
17
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
22
19
10
10
10
9
9
8
8
8
7
7
7
6
6
6
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
334
130
126
105
87
60
51
14
11
8
7
7
4
4
4
4
4
3
3
2
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand THE™
Immunogen A synthetic peptide (DYKDDDDK) coupled - KLH
Sequence DYKDDDDK
Clone 5A8E5
Isotype IgG
Specificity THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Characteristics Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Purification Protein A affinity column
Target Type Tag
Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Supplier Images
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1.N-terminal DYKDDDDK-fusion protein (400 ng) Lane 2.C-terminal DYKDDDD...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Loading: Lane 1-3 Multiple Tag (Purified) (ABIN1536315, 400 ng, 80 ng,16 ng) Lane 4. ...
 image for anti-DYKDDDDK Tag antibody (ABIN387700) Lane1: 1 µL E-coli lysate with DYKDDDDK-tag protein (MW: 57 kDa) and 2 µg Mouse Anti-...
Product cited in: Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." in: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, 2014 (PubMed).

Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." in: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, 2013 (PubMed).

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