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His Tag antibody (Biotin)

WB, ELISA Host: Mouse Monoclonal 6G2A9 Biotin
Catalog No. ABIN387702
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 92
    • 56
    • 12
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    • 1
    Mouse
    Clonality
    • 105
    • 69
    • 10
    Monoclonal
    Conjugate
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    This His Tag antibody is conjugated to Biotin
    Application
    • 152
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    Western Blotting (WB), ELISA
    Brand
    THE™
    Sequence
    HHHHHH
    Specificity
    THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
    Characteristics
    The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.
    Purification
    Protein A affinity column
    Immunogen
    A synthetic peptide (HHHHHH) coupled - KLH
    Clone
    6G2A9
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.02-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wegman, Geuze, van der Helm, Cumhur Öner, Dhert, Alblas: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).

    Ota, Doyle-Cooper, Cooper, Huber, Falkowska, Doores, Hangartner, Le, Sok, Jardine, Lifson, Wu, Mascola, Poignard, Binley, Chakrabarti, Schief, Wyatt, Burton, Nemazee: "Anti-HIV B Cell lines as candidate vaccine biosensors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).

  • Target
    His Tag
    Abstract
    His Tag Products
    Target Type
    Tag
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