C-Myc-Tag antibody

Antigen: C-Myc-Tag

Clonality: Monoclonal (2G8D5)

Host: Mouse

Application: ELISA, Western Blotting (WB), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Certificates: ISO 9001:2008

Catalog no.: ABIN387704

Additional Information

Immunogen: A synthetic peptide EQKLISEEDL coupled to KLH

Format: Purified

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: 2G8D5

Description: The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Specificity: Anti-c-Myc-tag Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05 ug/ml-0.2 ug/ml Western blot: 0.1 ug/ml-1 ug/ml Western blot using ONE-HOUR Western Kit: For quick results, Antibodies-Online ONE-HOUR Western Kit L00205C is recommended. 0.9 ug of this antibody is mixed with diluted WB solution (dilute 1.5 ml WB with 1.5 ml of PBST).Immunoprecipitation (IP): 1 ug/mlImmunofluorescent staining: 1 ug/ml Other applications: user-optimized

Concentration: 40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide and 100 µg, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Protein A affinity column

Storage: Anti-c-Myc-tag Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Multiple Tag Cell Lysate (Antibodies-Online, M0100)Primary antibody:A. 1 µg/ml Anti-c-Myc-tag Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396860)B. 1 µg/ml Anti-c-Myc-tag Monoclonal Antibody (Mouse) (From competitor A)Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398387, 1:10,000)

Loading:1. Lysate that plasmid containing GST-c-Myc transfected in 293 cells2. Lysate that plasmid containing c-Myc-GST transfected in 293 cellsPrimary antibody:A. 1 µg/ml Mouse Anti-c-Myc-tag Monoclonal Antibody (Antibodies-Online, ABIN396860)B. 1 µg/ml Mouse Anti c-Myc Monoclonal Antibody (From competitor A)Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398387, 1:10,000)

Lane 1-2: Multiplex tag cell lysate (Antibodies-Online, M0100)NC: 293 cell lysatePrimary antibody: Anti-c-Myc-tag Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396860)The Western was performed using One-Step Western Blot Kit (Antibodies-Online, L00205) with 0.9 µg of the antibody added to diluted WB solution (dilute 1.5 ml of WB with 1.5 ml of PBST)