alpha Tubulin antibody

Catalog No. ABIN387714

Product Details anti-alpha Tubulin Antibody

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Anti-alpha-tubulin Monoclonal Antibody (Mouse) reacts with mouse, human, hamster, monkey, and pig alpha-tubulin. This product has not yet been tested in other species.

Cross-Reactivity (Details): Cross reactivity in other species has not yet been tested.

Purification: Protein A affinity column

Immunogen: A synthetic peptide (ALEKDYEEVGVDSVE) coupled - KLH

Clone: 1F4E3

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunofluorescent staining: 1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-alpha Tubulin antibody (ABIN387714)

Amita, Adachi, Alexenko, Sinha, Schust, Schulz, Roberts, Ezashi: "Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 13, pp. E1212-21, (2013) (PubMed).

Chen, Cheng, Liu, King, Peng, Zhang, Wang, Shresta, Schooley, Liu: "Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses." in: Antiviral research, Vol. 99, Issue 3, pp. 371-82, (2013) (PubMed).

Kuo, Wang, Lee, Iwasaki, Duque-Afonso, Wong, Lin, Figueroa, Su, Lemischka, Cleary: "Epigenetic roles of MLL oncoproteins are dependent on NF-?B." in: Cancer cell, Vol. 24, Issue 4, pp. 423-37, (2013) (PubMed).

Lakshmana, Hayes, Bennett, Bianchi, Reddy, Koo, Kang: "Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 5, pp. 2072-83, (2012) (PubMed).

Schmid, Lehmann, Lucius, Gupta: "Apicomplexan parasite, Eimeria falciformis, co-opts host tryptophan catabolism for life cycle progression in mouse." in: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20197-207, (2012) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha Tubulin (TUBA1 Products)

Background: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to alpha-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.

Pathways: Microtubule Dynamics