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His Tag antibody (FITC)

WB, ELISA Host: Mouse Monoclonal 6G2A9 FITC
Catalog No. ABIN387722
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 92
    • 56
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    Mouse
    Clonality
    • 105
    • 69
    • 10
    Monoclonal
    Conjugate
    • 91
    • 22
    • 13
    • 12
    • 4
    • 3
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    This His Tag antibody is conjugated to FITC
    Application
    • 152
    • 102
    • 38
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    • 17
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    • 2
    • 1
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    Western Blotting (WB), ELISA
    Brand
    THE™
    Sequence
    HHHHHH
    Specificity
    THETM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
    Characteristics
    This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
    Purification
    Protein A affinity column
    Immunogen
    A synthetic peptide HHHHHH coupled - KLH
    Clone
    6G2A9
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Immunofluorescence (IF): 0.1-2 µg/mL
    Flow cytometry: 0.1-2 µg for 1 x 10^6 cells
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    1 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Yang, Wahlmüller, Sarg, Furtmüller, Geiger: "A+-helix of protein C inhibitor (PCI) is a cell-penetrating peptide that mediates cell membrane permeation of PCI." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 3081-91, (2015) (PubMed).

    Jarmander, Janoschek, Lundh, Larsson, Gustavsson: "Process optimization for increased yield of surface-expressed protein in Escherichia coli." in: Bioprocess and biosystems engineering, Vol. 37, Issue 8, pp. 1685-93, (2014) (PubMed).

    An, Budd, Kanost, Michel: "Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes." in: Cellular and molecular life sciences : CMLS, Vol. 68, Issue 11, pp. 1929-39, (2011) (PubMed).

    Oliveira, Lindblad: "Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 15, pp. 3804-14, (2011) (PubMed).

    Li, Wang, Zhou, Yang, Bhaskar, Hu, Sun, Miao: "Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system." in: Molecular biology reports, Vol. 37, Issue 8, pp. 3721-8, (2010) (PubMed).

  • Target
    His Tag
    Abstract
    His Tag Products
    Target Type
    Tag
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