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DYKDDDDK Tag antibody (FITC)

IF, FACS Host: Mouse Monoclonal 5A8E5 FITC
Catalog No. ABIN387724
  • Target See all DYKDDDDK Tag products
    DYKDDDDK Tag
    Reactivity
    Please inquire
    Host
    • 57
    • 52
    • 8
    • 6
    • 6
    Mouse
    Clonality
    • 65
    • 63
    Monoclonal
    Conjugate
    • 46
    • 15
    • 9
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DYKDDDDK Tag antibody is conjugated to FITC
    Application
    • 80
    • 62
    • 29
    • 23
    • 19
    • 16
    • 12
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS)
    Brand
    THE™
    Sequence
    DYKDDDDK
    Specificity
    THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
    Characteristics
    This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
    Purification
    Protein A affinity column
    Immunogen
    A synthetic peptide DYKDDDDK coupled - KLH
    Clone
    5A8E5
    Isotype
    IgG
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Immunofluorescence (IF): 0.5-2 µg/mL
    Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    0.5 mg/mL
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, (2012) (PubMed).

  • Target
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag Products
    Target Type
    Tag
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