DYKDDDDK Tag antibody (FITC)

Details for Product No. ABIN387724, Supplier: Log in to see
Antigen
Reactivity
Tag
240
101
39
18
18
9
8
7
4
3
2
1
1
1
Host
Mouse
173
110
34
34
17
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
38
20
16
8
8
8
8
6
6
5
5
5
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
290
131
126
103
85
58
42
14
11
8
7
7
4
4
4
4
3
3
3
2
1
1
1
1
1
1
Options
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Brand THE™
Immunogen A synthetic peptide DYKDDDDK coupled - KLH
Sequence DYKDDDDK
Clone 5A8E5
Isotype IgG
Specificity THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
Characteristics This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Purification Protein A affinity column
Target Type Tag
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/mL
Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Supplier Images
Flow Cytometry (FACS) image for anti-DYKDDDDK Tag antibody (FITC) (ABIN387724) Flow cytometric analysis of transfected HEK-293 cell expressing HA-tagged protein usi...
Product cited in: Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, 2012 (PubMed).