Myc Tag antibody

Details for Product No. ABIN387746, Supplier: Log in to see
Antigen
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Mouse
160
60
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
215
116
78
53
48
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Options
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Immunogen KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Sequence EQKLISEEDL
Clone 9E10
Isotype IgG1
Specificity KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
Synonyms: Tag from c-Myc protein
Molecular Weight 48 kDA
Research Area Tags/Labels
Application Notes WB = 1:2, 000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
 image for anti-Myc Tag antibody (ABIN387746) Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was ...
Product cited in: Wang, Zhang, Dou, Zhang, Chen, Yu, Ren: "Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis." in: PLoS genetics, Vol. 11, Issue 4, pp. e1005091, 2015 (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, 2015 (PubMed).

Hu, Yu, Zhou, Li, Xia, Luo, Wu: "A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3." in: Molecular medicine reports, Vol. 9, Issue 1, pp. 173-9, 2013 (PubMed).

Liu, Yan, Liu, Hong, Sun, Shi, Huang, Fang: "Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies." in: Plant physiology, Vol. 163, Issue 1, pp. 108-17, 2013 (PubMed).

Chu, Loughlin, Gaur, SenBanerjee, Jacob, Monson, Kent, Oranu, Ding, Ukomadu, Sadler: "UHRF1 phosphorylation by cyclin A2/cyclin-dependent kinase 2 is required for zebrafish embryogenesis." in: Molecular biology of the cell, Vol. 23, Issue 1, pp. 59-70, 2012 (PubMed).

Chu, Mir, Gao, Rosa, Monson, Sharma, Steet, Freeze, Lehrman, Sadler: "A zebrafish model of congenital disorders of glycosylation with phosphomannose isomerase deficiency reveals an early opportunity for corrective mannose supplementation." in: Disease models & mechanisms, Vol. 6, Issue 1, pp. 95-105, 2012 (PubMed).

Hakozaki, Yoda, Tohmonda, Furukawa, Hikata, Uchikawa, Takaishi, Matsumoto, Chiba, Horiuchi, Toyama: "Receptor activator of NF-kappaB (RANK) ligand induces ectodomain shedding of RANK in murine RAW264.7 macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 5, pp. 2442-8, 2010 (PubMed).

Satow, Shitashige, Jigami, Honda, Ono, Hirohashi, Yamada: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, 2010 (PubMed).

Kim, Kang, Nagaraj, Blancaflor, Mysore, Chapman: "Mutations in Arabidopsis fatty acid amide hydrolase reveal that catalytic activity influences growth but not sensitivity to abscisic acid or pathogens." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34065-74, 2009 (PubMed).

Kobayashi, Barnard, Said, Hong-Gonzalez, Corman, Ku, Doan, Gui, Elashoff, Cohen, Aronson: "Effect of low-fat diet on development of prostate cancer and Akt phosphorylation in the Hi-Myc transgenic mouse model." in: Cancer research, Vol. 68, Issue 8, pp. 3066-73, 2008 (PubMed).

Zemskov, Mikhailenko, Strickland, Belkin: "Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1." in: Journal of cell science, Vol. 120, Issue Pt 18, pp. 3188-99, 2007 (PubMed).

Hang, Zemskov, Lorand, Belkin: "Identification of a novel recognition sequence for fibronectin within the NH2-terminal beta-sandwich domain of tissue transglutaminase." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23675-83, 2005 (PubMed).