His Tag antibody

Details for Product No. ABIN387750, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Mouse
434
184
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
554
386
167
107
99
77
69
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produce this monoclonal antibody.
Sequence HHHHHH
Clone 6AT18
Isotype IgG1
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Molecular Weight 1000 Da
Gene ID 12406
Research Area Tags/Labels
Application Notes Suggested dilutions:
WB = 1:1,000
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
 image for anti-His Tag antibody (ABIN387750) HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS m...
Product cited in: Kim, Pai, Sato, Person, Nakamura, Macdonald: "Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression." in: PLoS genetics, Vol. 11, Issue 2, pp. e1004992, 2015 (PubMed).

Grigoroudis, McInnes, Premnath, Kontopidis: "Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones." in: Protein expression and purification, Vol. 113, pp. 8-16, 2015 (PubMed).

Yarosh, Tapescu, Thompson, Qiu, Mallory, Fu, Lynch: "TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells." in: Nucleic acids research, Vol. 43, Issue 18, pp. 9006-16, 2015 (PubMed).

Lv, Lei, Hong, Wang, Zhang, Zhou, Sun, Ma, Shen, Zhu: "Venom allergen 5 is Associated With Deltamethrin Resistance in Culex pipiens pallens (Diptera: Culicidae)." in: Journal of medical entomology, Vol. 52, Issue 4, pp. 672-82, 2015 (PubMed).

Bhandari, Lopez-Sanchez, To, Lo, Aznar, Leyme, Gupta, Niesman, Maddox, Garcia-Marcos, Farquhar, Ghosh: "Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 35, pp. E4874-83, 2015 (PubMed).

Shi, Zhang, Guo, Zhang, Hu, Xi, Miao, Li: "131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma." in: Oncology reports, 2015 (PubMed).

Liu, Novick: "Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p." in: The Journal of cell biology, Vol. 207, Issue 1, pp. 59-72, 2014 (PubMed).

Hufnagel, DePas, Chapman: "The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli." in: Journal of bacteriology, Vol. 196, Issue 21, pp. 3690-9, 2014 (PubMed).

Faure, Nagarajan, Hwang, Montgomery, Khan, John, Koulen, Blancaflor, Chapman: "Synthesis of phenoxyacyl-ethanolamides and their effects on fatty acid amide hydrolase activity." in: The Journal of biological chemistry, Vol. 289, Issue 13, pp. 9340-51, 2014 (PubMed).

Malmirchegini, Sjodt, Shnitkind, Sawaya, Rosinski, Newton, Klebba, Clubb: "Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes." in: The Journal of biological chemistry, Vol. 289, Issue 50, pp. 34886-99, 2014 (PubMed).

Wang, Xiang, Zhang, Chen, Zhong, Wang: "Development of a functional antibody by using a green fluorescent protein frame as the template." in: Applied and environmental microbiology, Vol. 80, Issue 14, pp. 4126-37, 2014 (PubMed).

Hsu, Motiwala, Roy, Claus, Mustafa, Plass, Freitas, Ghoshal, Jacob: "Methylation of the PTPRO gene in human hepatocellular carcinoma and identification of VCP as its substrate." in: Journal of cellular biochemistry, Vol. 114, Issue 8, pp. 1810-8, 2013 (PubMed).

Shen, Yuan, Hutagalung, Verma, Kümmel, Wu, Reinisch, McNew, Novick: "The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p." in: The Journal of cell biology, Vol. 202, Issue 3, pp. 509-26, 2013 (PubMed).

Narayanan, Gabrieli, Leung, Elguindy, Glaser, Saju, Sinha, Nakamaru-Ogiso: "Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 14310-9, 2013 (PubMed).

Feltcher, Gibbons, Ligon, Braunstein: "Protein export by the mycobacterial SecA2 system is determined by the preprotein mature domain." in: Journal of bacteriology, Vol. 195, Issue 4, pp. 672-81, 2013 (PubMed).

Anderson, Miller, Fierobe, Clubb: "Recombinant Bacillus subtilis that grows on untreated plant biomass." in: Applied and environmental microbiology, Vol. 79, Issue 3, pp. 867-76, 2013 (PubMed).

Rytinki, Kaikkonen, Sutinen, Palvimo: "Analysis of androgen receptor SUMOylation." in: Methods in molecular biology (Clifton, N.J.), Vol. 776, pp. 183-97, 2011 (PubMed).

Ye, Selby, Ozturk, Annayev, Sancar: "Biochemical analysis of the canonical model for the mammalian circadian clock." in: The Journal of biological chemistry, Vol. 286, Issue 29, pp. 25891-902, 2011 (PubMed).

Yilmaz, Sancar, Kemp: "Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates." in: PLoS ONE, Vol. 6, Issue 7, pp. e22986, 2011 (PubMed).

Terasawa, Toya, Motegi, Mana, Nakamura, Sugimoto: "Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 11, pp. 1145-57, 2010 (PubMed).

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