Biotin-EpCAM antibody (Biotin)

Antigen: Biotin-EpCAM

Clonality: Monoclonal (68CT112)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387762

Additional Information

Gene ID: 4072

UniProt: NP_002345

Immunogen: This monoclonal antibody is generated from mice immunized with a purified recombinant EpCAM protein.

Isotype: IgG2a

Clone: 68CT112

Description: Other names: CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, KS1/4, KSA, M4S1, MIC18, MK-1, TACST-1, TACSTD1, TROP1, hEGP-2

Characteristics: Biotinalyted Mouse Monoclonal Antibody (Mab)

Specificity: This monoclonal antibody is generated from mice immunized with a purified recombinant EpCAM protein.

Molecular Weight: 40 kDA

Comments:

Background: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Application Details

Application Notes: ELISA~~1:1,000-2,500 Flow cytometry~~1:1,000-1:7,500 Immunoprecipitation 5 ?g per test

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure, Cancer

Restrictions: For Research Use only

Publications

Product: Lu, Lu, Liao et al.: "Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells." in: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 8719-32, 2010 (PubMed).

Raffel, Eisenberger, Cupisti et al.: "Increased EpCAM expression in malignant insulinoma: potential clinical implications." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 162, Issue 2, pp. 391-8, 2010 (PubMed).

Lugli, Iezzi, Hostettler et al.: "Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer." in: British journal of cancer, Vol. 103, Issue 3, pp. 382-90, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Jiang, Zhang, Li et al.: "A non-synonymous polymorphism Thr115Met in the EpCAM gene is associated with an increased risk of breast cancer in Chinese population." in: Breast cancer research and treatment, Vol. 126, Issue 2, pp. 487-95, 2011 (PubMed).