Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1) antibody

Antigen: Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1)

Synonyms: MG44, KMT1A, SUV39H

Clonality: Monoclonal (42AT239-96-72)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387778

Additional Information

Alternative name: SUV39H1

Gene ID: 6839

UniProt: NP_003164

Immunogen: This SUV39H1 antibody was raised using purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Isotype: IgG1

Clone: 42AT239-96-72

Description: Other names: Histone-lysine N-methyltransferase, H3 lysine-9 specific 1, Suppressor of variegation 3-9 homolog 1,, KMT1A

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This SUV39H1 antibody was raised using purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Molecular Weight: 47907 DA

Comments:

Background: This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western analysis of extracts from 293 cells using SUV39H1 antibody.

Publications

Product: Spensberger, Delwel: "A novel interaction between the proto-oncogene Evi1 and histone methyltransferases, SUV39H1 and G9a." in: FEBS letters, Vol. 582, Issue 18, pp. 2761-7, 2008 (PubMed).

Li, Chen, Kabra et al.: "Inhibition of SUV39H1 methyltransferase activity by DBC1." in: The Journal of biological chemistry, Vol. 284, Issue 16, pp. 10361-6, 2009 (PubMed).

Cherrier, Suzanne, Redel et al.: "p21(WAF1) gene promoter is epigenetically silenced by CTIP2 and SUV39H1." in: Oncogene, Vol. 28, Issue 38, pp. 3380-9, 2009 (PubMed).

Goyama, Nitta, Yoshino et al.: "EVI-1 interacts with histone methyltransferases SUV39H1 and G9a for transcriptional repression and bone marrow immortalization." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 24, Issue 1, pp. 81-8, 2010 (PubMed).

Chen, Li, Zwolinska et al.: "MDM2 recruitment of lysine methyltransferases regulates p53 transcriptional output." in: The EMBO journal, Vol. 29, Issue 15, pp. 2538-52, 2010 (PubMed).