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Nanog Homeobox (NANOG) antibody

Details for Product No. ABIN387791, Supplier: Log in to see
Antigen
  • 2410002E02Rik
  • ecat4
  • ENK
Reactivity
Human
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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35
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14
6
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Supplier
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Immunogen This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Clone 60CT77-1-1
Isotype IgG1, kappa
Specificity This NANOG monoclonal antibody is generated from mouse immunized with NANOG recombinant protein.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name NANOG (NANOG Antibody Abstract)
Background Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).
Synonyms: Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog
Molecular Weight 34620 DA
Gene ID 79923
NCBI Accession NP_079141, XP_002344676
UniProt Q9H9S0
Research Area Stem Cells, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:100-250, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.49 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Nanog Homeobox (NANOG) antibody (ABIN387791) Western blot analysis of anti-NANOG monoclonal antibody (ABIN387791) by NONAG recombi...
Background publications Trubiani, Zalzal, Paganelli, Marchisio, Giancola, Pizzicannella, Bühring, Piattelli, Caputi, Nanci: "Expression profile of the embryonic markers nanog, OCT-4, SSEA-1, SSEA-4, and frizzled-9 receptor in human periodontal ligament mesenchymal stem cells." in: Journal of cellular physiology, Vol. 225, Issue 1, pp. 123-31, 2010 (PubMed).

Po, Ferretti, Miele, De Smaele, Paganelli, Canettieri, Coni, Di Marcotullio, Biffoni, Massimi, Di Rocco, Screpanti, Gulino: "Hedgehog controls neural stem cells through p53-independent regulation of Nanog." in: The EMBO journal, Vol. 29, Issue 15, pp. 2646-58, 2010 (PubMed).

Zbinden, Duquet, Lorente-Trigos, Ngwabyt, Borges, Ruiz i Altaba: "NANOG regulates glioma stem cells and is essential in vivo acting in a cross-functional network with GLI1 and p53." in: The EMBO journal, Vol. 29, Issue 15, pp. 2659-74, 2010 (PubMed).

Moretto-Zita, Jin, Shen, Zhao, Briggs, Xu: "Phosphorylation stabilizes Nanog by promoting its interaction with Pin1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13312-7, 2010 (PubMed).