Beclin 1, Autophagy Related (BECN1) antibody

Antigen: Beclin 1, Autophagy Related (BECN1)

Synonyms: ATG6, VPS30, beclin1, MGC6843, 4921513J16Rik, 5430417M23Rik, MGC73097, zgc:73097, BECN1, becn1, MGC80672, MGC126978, atg6, vps30, Beclin-1, DKFZp459C0719

Clonality: Monoclonal (15CT26-4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387797

Additional Information

Alternative name: Beclin 1

Gene ID: 8678

UniProt: Q14457

Immunogen: This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.

Format: Ascites

Isotype: IgG3 lambda

Clone: 15CT26-4

Description: Other names: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.

Molecular Weight: 51765 DA

Comments:

Background: Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:500-1000 Immunohistochemistry~~1:50-1:200

Concentration: Crude ascites

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Autophagy

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of anti-BECN1 Mab (ABIN387797) in HepG2, 293, Jurkat and Hela cell line lysates (35ug/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution). (RIGHT)Western blot analysis of anti-BECN1 Mab (CA080116A) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution). Breast CA section stained with Autophagy Beclin 1 Antibody Cat. # ABIN387797 at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana.

anti-Beclin 1, Autophagy Related (BECN1) antibody (Image 2)

Publications

Product: Sun, Liu, Jin et al.: "Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells." in: Cancer letters, Vol. 294, Issue 2, pp. 204-10, 2010 (PubMed).

Li, Chen, Wu et al.: "Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors." in: BMC cancer, Vol. 10, pp. 98, 2010 (PubMed).

Jaeger, Pickford, Sun et al.: "Regulation of amyloid precursor protein processing by the Beclin 1 complex." in: PLoS ONE, Vol. 5, Issue 6, pp. e11102, 2010 (PubMed).

Niu, Cheng, Ren et al.: "Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis." in: FEBS letters, Vol. 584, Issue 16, pp. 3519-24, 2010 (PubMed).

Metzger, Saukko, Van Che et al.: "Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7." in: Human genetics, Vol. 128, Issue 4, pp. 453-9, 2010 (PubMed).