SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody

Antigen: SRY (Sex Determining Region Y)-Box 2 (SOX2)

Synonyms: ANOP3, MCOPS3, MGC2413, RGD1565646, SOX2, MGC148683, lcc, ysb, Sox-2

Clonality: Monoclonal (57CT23-3-4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387799

Additional Information

Alternative name: SOX2

Gene ID: 6657

UniProt: P48431

Immunogen: Human SOX2 recombinant protein is used to produce this monoclonal antibody.

Isotype: IgG1

Clone: 57CT23-3-4

Description: Other names: Transcription factor SOX-2

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: Human SOX2 recombinant protein is used to produce this monoclonal antibody.

Molecular Weight: 34310 DA

Comments:

Background: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:10000 Western blotting~~ 1:200~2000 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:50~100

Concentration: Ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-SOX2 Monoclonal Antibody(Cat.#ABIN387799) by human SOX2 recombinant protein cell line lysate (35ug/lane). SOX2 (His tagged) (arrow) was detected using the purified Mab. Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (ascites)(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (Image 2)

Publications

Product: Reis, Tyler, Schneider et al.: "Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes." in: Molecular vision, Vol. 16, pp. 768-73, 2010 (PubMed).

Gonzalez-Rodriguez, Pelcastre, Tovilla-Canales et al.: "Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases." in: The British journal of ophthalmology, Vol. 94, Issue 8, pp. 1100-4, 2010 (PubMed).

Ge, Zhou, Chen et al.: "Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells." in: Biochemical and biophysical research communications, Vol. 397, Issue 4, pp. 711-7, 2010 (PubMed).

Sholl, Barletta, Yeap et al.: "Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma." in: The American journal of surgical pathology, Vol. 34, Issue 8, pp. 1193-8, 2010 (PubMed).

Zhang, Efendic, Brismar et al.: "Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus." in: BMC medical genetics, Vol. 11, pp. 116, 2010 (PubMed).