EPH Receptor B3 (EPHB3) antibody

Antigen: EPH Receptor B3 (EPHB3)

Synonyms: ETK2, HEK2, TYRO6, EK10, CEK10, EPHB3, tck, MGC83457

Clonality: Monoclonal (50CT97-1-9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387813

Additional Information

Alternative name: EPHB3

Gene ID: 2049

UniProt: P54753

Immunogen: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Isotype: IgG1 kappa

Clone: 50CT97-1-9

Description: Other names: Ephrin type-B receptor 3, Tyrosine-protein kinase receptor HEK-2

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Molecular Weight: 110286 DA

Comments:

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:4000 Western blotting~~1:2000

Concentration: Crude ascites

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of anti-EPHB3 Monoclonal Antibody (Cat.#ABIN387813) by EPHB3 recombinant protein (Fragment). EPHB3 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)

Publications

Product: Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Vieira, McHenry, Daack-Hirsch et al.: "Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 10, Issue 9, pp. 668-74, 2008 (PubMed).

Chiu, Chang, Ting et al.: "Over-expression of EphB3 enhances cell-cell contacts and suppresses tumor growth in HT-29 human colon cancer cells." in: Carcinogenesis, Vol. 30, Issue 9, pp. 1475-86, 2009 (PubMed).

Kang, Koo, Kwon et al.: "Identification of novel candidate target genes, including EPHB3, MASP1 and SST at 3q26.2-q29 in squamous cell carcinoma of the lung." in: BMC cancer, Vol. 9, pp. 237, 2009 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).