|+1 404 474 4654|
|+1 888 205 9894 (TF)|
EPH Receptor B3 (EPHB3) antibody
|Synonyms||ETK2, HEK2, TYRO6, EK10, CEK10, EPHB3, tck, MGC83457|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 ml (Crude ascites)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Description||Other names: Ephrin type-B receptor 3, Tyrosine-protein kinase receptor HEK-2|
|Characteristics||Mouse Monoclonal Antibody (Mab)|
|Specificity||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Molecular Weight||110286 DA|
Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.
|Application Notes||The suggested dilution is: ELISA~~1:4000 Western blotting~~1:2000|
|Buffer||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology|
|Restrictions||For Research Use only|
|Western blot analysis of anti-EPHB3 Monoclonal Antibody (Cat.#ABIN387813) by EPHB3 recombinant protein (Fragment). EPHB3 (Fragment) protein (arrow) was detected using the ascites Mab. (1:2000)|
Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).
Vieira, McHenry, Daack-Hirsch et al.: "Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 10, Issue 9, pp. 668-74, 2008 (PubMed).
Chiu, Chang, Ting et al.: "Over-expression of EphB3 enhances cell-cell contacts and suppresses tumor growth in HT-29 human colon cancer cells." in: Carcinogenesis, Vol. 30, Issue 9, pp. 1475-86, 2009 (PubMed).
Kang, Koo, Kwon et al.: "Identification of novel candidate target genes, including EPHB3, MASP1 and SST at 3q26.2-q29 in squamous cell carcinoma of the lung." in: BMC cancer, Vol. 9, pp. 237, 2009 (PubMed).
Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).
|Hosts||Rabbit (32), Mouse (13), Goat (1)|
|Reactivities||Human (45), Mouse (Murine) (22), Cow (Bovine) (18), Dog (Canine) (18), Rat (Rattus) (2)|
|Applications||Western Blotting (WB) (31), ELISA (23), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (IHC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||N-Term (3), AA 30-554 (1), AA 39-212 (1), C-Term (1), Center (1), Extracellular Domain (1)|