conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody

Antigen: conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)

Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053

Clonality: Monoclonal (61AT277)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387825

Additional Information

Alternative name: IKK alpha

UniProt: O15111

Immunogen: This antibody was raised using purified His-tagged recombinant full length human IKK alpha.

Isotype: IgG1

Clone: 61AT277

Description: Other names: Inhibitor of nuclear factor kappa-B kinase alpha subunit, I kappa-B kinase alpha, IkBKA, IKK-A, IkappaB kinase, I-kappa-B kinase 1, IKK1, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NFkappaB inhibitor kinase alpha, NFKBIKA

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: This IKK alpha antibody was raised using purified His-tagged recombinant full length human IKK alpha.

Comments:

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Application Details

Application Notes: The suggested dilution will be: ELISA 1:1,000 Western blotting 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-IKKalpha Mab (Cat. #ABIN387825) is used in Western blot to detect IKKalpha in HeLa cell lysate.

Publications

Product: Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Rotman, Koh, Zmuda et al.: "The association of genetic variability in patatin-like phospholipase domain-containing protein 3 (PNPLA3) with histological severity of nonalcoholic fatty liver disease." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 3, pp. 894-903, 2010 (PubMed).

Li, Morgan, Choksi et al.: "MicroRNAs modulate the noncanonical transcription factor NF-kappaB pathway by regulating expression of the kinase IKKalpha during macrophage differentiation." in: Nature immunology, Vol. 11, Issue 9, pp. 799-805, 2010 (PubMed).