HA-Tag antibody

Details for Product No. ABIN387848, Supplier: Log in to see
Antigen
Reactivity
Tag
450
2
1
1
1
1
1
Host
Rabbit
179
178
40
34
20
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
43
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Western Blotting (WB)
360
201
116
108
81
70
53
40
15
13
11
7
6
5
4
4
4
4
3
2
2
1
1
1
1
1
1
Options
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Immunogen KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Sequence YPYDVPDYA
Clone RB0411
Isotype Ig
Specificity KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Synonyms: Tag from influenza hemagglutinin protein
Molecular Weight DA
Research Area Tags/Labels
Application Notes WB = 1:1000, IF = 1:10-50, CHIP = 1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN387848) Western blot analysis of anti-HA tag Pab (ABIN387848)in HA-tagged recombinant protein...
Immunohistochemistry (IHC) image for anti-HA-Tag antibody (ABIN387848) GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 f...
Immunohistochemistry (IHC) image for anti-HA-Tag antibody (ABIN387848) ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune ...
Product cited in: Koo, Yue, Deng, Khuri, Sun: "mTOR Complex 2 Stabilizes Mcl-1 Protein by Suppressing Its Glycogen Synthase Kinase 3-Dependent and SCF-FBXW7-Mediated Degradation." in: Molecular and cellular biology, Vol. 35, Issue 13, pp. 2344-55, 2015 (PubMed).

Han, Guan, Wang, Hatzoglou, Mu: "L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated ?1(D219N) Subunits of GABAA Receptors." in: ACS chemical biology, 2015 (PubMed).

Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, 2015 (PubMed).

Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, 2015 (PubMed).

Oh, Yue, Wang, Tong, Chen, Khuri, Sun: "Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling." in: Oncotarget, 2015 (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, 2015 (PubMed).

Zhao, Yue, Khuri, Sun: "mTOR complex 2 is involved in regulation of Cbl-dependent c-FLIP degradation and sensitivity of TRAIL-induced apoptosis." in: Cancer research, Vol. 73, Issue 6, pp. 1946-57, 2013 (PubMed).

Oh, Yue, Zhou, Balko, Black, Owonikoko, Khuri, Sun: "Oncogenic Ras and B-Raf proteins positively regulate death receptor 5 expression through co-activation of ERK and JNK signaling." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 257-67, 2012 (PubMed).

Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, 2012 (PubMed).

Zhao, Yue, Lonial, Khuri, Sun: "The NEDD8-activating enzyme inhibitor, MLN4924, cooperates with TRAIL to augment apoptosis through facilitating c-FLIP degradation in head and neck cancer cells." in: Molecular cancer therapeutics, Vol. 10, Issue 12, pp. 2415-25, 2011 (PubMed).

Fan, Li, Yue, Khuri, Sun: "The eIF4E/eIF4G interaction inhibitor 4EGI-1 augments TRAIL-mediated apoptosis through c-FLIP Down-regulation and DR5 induction independent of inhibition of cap-dependent protein translation." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 4, pp. 346-56, 2010 (PubMed).

Guo, Wang, Wang, Huang, Liang, Chen: "Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis." in: Plant & cell physiology, Vol. 50, Issue 9, pp. 1681-94, 2009 (PubMed).

Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, 2009 (PubMed).

Lin, Liu, Yue, Benbrook, Berlin, Khuri, Sun: "Involvement of c-FLIP and survivin down-regulation in flexible heteroarotinoid-induced apoptosis and enhancement of TRAIL-initiated apoptosis in lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 11, pp. 3556-65, 2008 (PubMed).

Wang, Kwak, Zeng, Ellis, Chen, Schiefelbein, Chen: "TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis." in: Development (Cambridge, England), Vol. 134, Issue 21, pp. 3873-82, 2007 (PubMed).

Zemskov, Mikhailenko, Strickland, Belkin: "Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1." in: Journal of cell science, Vol. 120, Issue Pt 18, pp. 3188-99, 2007 (PubMed).

Liu, Yue, Schönthal, Khuri, Sun: "Cellular FLICE-inhibitory protein down-regulation contributes to celecoxib-induced apoptosis in human lung cancer cells." in: Cancer research, Vol. 66, Issue 23, pp. 11115-9, 2006 (PubMed).