DYKDDDDK Tag antibody

Details for Product No. ABIN387849, Supplier: Log in to see
Antigen
Reactivity
Tag
233
121
65
18
18
16
9
7
4
3
2
1
1
1
Host
Rabbit
195
135
34
34
17
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
40
22
19
10
10
10
9
9
8
8
8
7
7
7
6
6
6
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
334
131
126
105
88
60
51
14
11
8
7
7
4
4
4
4
4
3
3
2
1
1
1
1
1
1
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Immunogen KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Sequence DYKDDDDK
Clone RB0493
Isotype Ig
Specificity KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target Type Tag
Background Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
Synonyms: Tag from influenza hemagglutinin protein
Molecular Weight DA
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DYKDDDDK Tag antibody (ABIN387849) Western blot analysis of anti-tag-DYKDDDDK.2x Pab (ABIN387849) in 12tag cell line lys...
Background publications Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).

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