DYKDDDDK Tag antibody

Details for Product No. ABIN387849, Supplier: Log in to see
Antigen
Reactivity
Tag
240
101
39
18
18
9
8
7
4
3
2
1
1
1
Host
Rabbit
174
109
34
34
17
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
38
21
16
8
8
8
8
6
6
5
5
5
5
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
289
131
127
103
85
58
43
14
11
8
7
7
4
4
4
4
3
3
3
2
1
1
1
1
1
1
Options
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Immunogen KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Sequence DYKDDDDK
Clone RB0493
Isotype Ig
Specificity KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Target Type Tag
Background Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1).
Synonyms: Tag from influenza hemagglutinin protein
Molecular Weight DA
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DYKDDDDK Tag antibody (ABIN387849) Western blot analysis of anti-tag-DYKDDDDK.2x Pab (ABIN387849) in 12tag cell line lys...
Background publications Chubet, Brizzard: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, 1996 (PubMed).