V5 Epitope Tag antibody

Details for Product No. ABIN387850, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Rabbit
221
134
37
9
5
4
3
1
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
29
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
342
266
123
92
71
65
53
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Sequence GKPIPNPLLG LDST
Clone RB0413
Isotype Ig
Specificity KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name V5-Tag
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Abgent's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
Molecular Weight DA
Research Area Tags/Labels
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
 image for anti-V5 Epitope Tag antibody (ABIN387850) Lanes: 1:non-transfected cells. 2:V5-tagged empty plasmid. 3:protein A (V5-tagged). 4...
Immunohistochemistry (IHC) image for anti-V5 Epitope Tag antibody (ABIN387850) Western blot analysis of anti-V5 Tag Antibody (ABIN387850) in 12tag protein (35 µg/la...
Background publications Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).