Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1) (C-Term), (AA 1145-1174) antibody

Details for Product No. ABIN387855
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Antigen
Synonyms EHMT1, G9a, 9230102N17Rik, D330003E03, Eu-HMTase1, GLP, GLP1, KMT1D, mKIAA1876, EUHMTASE1, FP13812, bA188C12.1
Epitope
C-Term, AA 1145-1174
(16), (12), (8), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(58), (2), (1), (1)
Host
Rabbit
(46), (11), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(52), (39), (6), (4), (2), (2), (2), (1)
Pubmed 2 references available
Quantity 400 μL
Options
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Catalog No. ABIN387855
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Immunogen This EHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1145-1174 AA from the C-terminal region of human EHMT1.
Clone RB13019
Isotype Ig
Specificity This EHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1121~1151 amino acids from the C-terminal region of human EHMT1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EHMT1
Background EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
Synonyms: Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP1, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, KIAA1876, KMT1D
Molecular Weight 138254 DA
Gene ID 79813
UniProt Q9H9B1
Research Area Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1) (C-Term), (AA 1145-1174) antibody Western blot analysis of EHMT1 (arrow) using rabbit polyclonal EHMT1 Antibody (C-term) (ABIN387855). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EHMT1 gene (Lane 2) (Origene Technologies).
Background publications Ogawa, Ishiguro, Gaubatz et al.: "A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells." in: Science (New York, N.Y.), Vol. 296, Issue 5570, pp. 1132-6, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

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