Kdm6b antibody (C-Term)

Catalog No. ABIN387863

Product Details anti-Kdm6b Antibody

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Binding Specificity: AA 1606-1641, C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: H

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1606-1641 amino acids from the C-terminal region of mouse JMJD3.

Clone: RB10081-RB10082

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-Kdm6b antibody (ABIN387863)

Pham, Yu, Walline, Muthukrishnan, Blum, Kaplan: "Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 2, pp. 902-11, (2013) (PubMed).

Das, Jung, Choi, Yoon, Kim, Chai: "Gene networking and inflammatory pathway analysis in a JMJD3 knockdown human monocytic cell line." in: Cell biochemistry and function, Vol. 30, Issue 3, pp. 224-32, (2012) (PubMed).

Solá, Xavier, Santos, Aranha, Morgado, Jepsen, Rodrigues: "p53 interaction with JMJD3 results in its nuclear distribution during mouse neural stem cell differentiation." in: PLoS ONE, Vol. 6, Issue 3, pp. e18421, (2011) (PubMed).

McLaughlin-Drubin, Crum, Münger: "Human papillomavirus E7 oncoprotein induces KDM6A and KDM6B histone demethylase expression and causes epigenetic reprogramming." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 5, pp. 2130-5, (2011) (PubMed).

Das, Jung, Chai: "The role of NF-kappaB and H3K27me3 demethylase, Jmjd3, on the anthrax lethal toxin tolerance of RAW 264.7 cells." in: PLoS ONE, Vol. 5, Issue 3, pp. e9913, (2010) (PubMed).

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: JMJD3 (Kdm6b Products)

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Molecular Weight: 176355

Gene ID: 216850

NCBI Accession: NP_001017426

UniProt: Q5NCY0

Pathways: Warburg Effect