Kdm6b antibody (AA 954-987)

Catalog No. ABIN387864

Product Details anti-Kdm6b Antibody

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Binding Specificity: AA 954-987

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kdm6b antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 954-987 amino acids from the Central region of mouse JMJD3.

Clone: RB10080

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Kdm6b antibody (ABIN387864)

Shaw, Martin: "Epigenetic reprogramming during wound healing: loss of polycomb-mediated silencing may enable upregulation of repair genes." in: EMBO reports, Vol. 10, Issue 8, pp. 881-6, (2009) (PubMed).

Target Details for Kdm6b

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: JMJD3 (Kdm6b Products)

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Molecular Weight: 176355

Gene ID: 216850

NCBI Accession: NP_001017426

UniProt: Q5NCY0

Pathways: Warburg Effect