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DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody

Details for Product No. ABIN387881, Supplier: Log in to see
Antigen
  • DNMT3A
  • DNMT3A2
  • M.HsaIIIA
  • MmuIIIA
Epitope
AA 457-486
41
22
16
15
15
14
12
8
7
7
7
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
200
55
35
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
186
16
2
2
1
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
13
11
11
8
8
8
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
170
97
55
23
14
11
11
8
6
4
1
Supplier
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Immunogen This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 AA from human Dnmt3a.
Clone RB01852
Isotype Ig
Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465~494 amino acids from the central region of human Dnmt3a.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Dnmt3a (DNMT3A Antibody Abstract)
Background CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Synonyms: DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA
Molecular Weight 101558 DA
Gene ID 1788
UniProt Q9Y6K1
Research Area Signaling, Protein Modifications, Cancer, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody (ABIN387881) Western blot analysis of anti-Dnmt3a Pab (ABIN387881) in T47-D cell lysate. Dnmt3a (A...
Western Blotting (WB) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody (ABIN387881) Lysates from thymus tissue after radiation were subjected to WB using antibody agains...
Immunohistochemistry (IHC) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody (ABIN387881) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Bing, Zhu, Yu et al.: "Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells." in: The Journal of biological chemistry, Vol. 289, Issue 47, pp. 32639-55, 2014 (PubMed).

Siddiqi, Terry, Matushansky: "Hiwi mediated tumorigenesis is associated with DNA hypermethylation." in: PLoS ONE, Vol. 7, Issue 3, pp. e33711, 2012 (PubMed).

Chen, Wang, Hu et al.: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, 2011 (PubMed).

Koturbash, Zemp, Kolb et al.: "Sex-specific radiation-induced microRNAome responses in the hippocampus, cerebellum and frontal cortex in a mouse model." in: Mutation research, Vol. 722, Issue 2, pp. 114-8, 2011 (PubMed).

Yamagata, Asada, Tamura et al.: "DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen." in: Human reproduction (Oxford, England), Vol. 24, Issue 5, pp. 1126-32, 2009 (PubMed).

Ishii, Kohu, Yamada et al.: "Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors." in: The Tohoku journal of experimental medicine, Vol. 212, Issue 2, pp. 177-90, 2007 (PubMed).

Koturbash, Boyko, Rodriguez-Juarez et al.: "Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1831-8, 2007 (PubMed).

Tryndyak, Muskhelishvili, Kovalchuk et al.: "Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis." in: Carcinogenesis, Vol. 27, Issue 8, pp. 1713-20, 2006 (PubMed).

Koturbash, Rugo, Hendricks et al.: "Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo." in: Oncogene, Vol. 25, Issue 31, pp. 4267-75, 2006 (PubMed).

Pogribny, Koturbash, Tryndyak et al.: "Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus." in: Molecular cancer research : MCR, Vol. 3, Issue 10, pp. 553-61, 2005 (PubMed).

Background publications Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Robertson, Uzvolgyi, Liang et al.: "The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors." in: Nucleic acids research, Vol. 27, Issue 11, pp. 2291-8, 1999 (PubMed).