Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 264-291), (C-Term) antibody

Details for Product No. ABIN387891
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Antigen
Synonyms xmbd3, MGC69548, MBD3, DKFZp459N1635, AI181826, AU019209
Epitope
AA 264-291, C-Term
(20), (15), (14), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(72), (23), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(57), (14), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(70), (31), (10), (8), (6), (4), (3), (2), (1)
Pubmed 1 reference available
Quantity 400 μL
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Immunogen This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 AA from the C-terminal region of human MBD3.
Clone RB02375
Isotype Ig
Specificity This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262~291 amino acids surrounding E279 of human MBD3.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MBD3 (MBD3 Antibody Abstract)
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Synonyms: Methyl-CpG-binding Domain 3
Molecular Weight 31789 DA
Gene ID 53615
UniProt O95983
Research Area Signaling, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 264-291), (C-Term) antibody Western blot analysis of MBD3 (arrow) using rabbit polyclonal MBD3 Antibody (C-term) ...
anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 264-291), (C-Term) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Validation Images
Catalog No. ABIN387891
291.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
291.50 $

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