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Details for Product No. ABIN387891

Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term), (AA 264-291) antibody

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Antigen
Synonyms AI181826, AU019209, xmbd3, MGC69548, MBD3, DKFZp459N1635
Epitope
»Alternatives C-Term, AA 264-291
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN387891
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 AA from the C-terminal region of human MBD3.
Clone RB02375
Isotype Ig
Specificity This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262~291 amino acids surrounding E279 of human MBD3.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MBD3
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Synonyms: Methyl-CpG-binding Domain 3
Molecular Weight 31789 DA
Gene ID 53615
UniProt O95983
Research Area Signaling, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes Recommend dilutions: WB : 1:1000,IHC : 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Alternatives for antigen "Methyl-CpG Binding Domain Protein 3 (MBD3)", type "Antibodies"
Hosts (34), (14), (1)
Reactivities (47), (17), (5), (1), (1), (1), (1), (1), (1), (1)
Applications (47), (16), (8), (5), (4), (4), (2), (1)
Epitopes (7), (5), (3), (3), (2), (1), (1), (1), (1)
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