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MBD3 antibody (C-Term)

MBD3 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB02375 unconjugated
Catalog No. ABIN387891
  • Target See all MBD3 Antibodies
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    Binding Specificity
    • 14
    • 9
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 264-291, C-Term
    Reactivity
    • 70
    • 28
    • 15
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 59
    • 9
    • 2
    Rabbit
    Clonality
    • 55
    • 15
    Polyclonal
    Conjugate
    • 42
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MBD3 antibody is un-conjugated
    Application
    • 46
    • 29
    • 10
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogen
    This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-291 amino acids from the C-terminal region of human MBD3.
    Clone
    RB02375
    Isotype
    Ig Fraction
    Top Product
    Discover our top product MBD3 Primary Antibody
  • Application Notes
    WB: 1:1000. IHC-P: 1:50~100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Expiry Date
    6 months
  • Target
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    Alternative Name
    MBD3 (MBD3 Products)
    Synonyms
    xmbd3 antibody, MGC69548 antibody, MBD3 antibody, DKFZp459N1635 antibody, AI181826 antibody, AU019209 antibody, methyl-CpG binding domain protein 3 S homeolog antibody, methyl-CpG binding domain protein 3 antibody, mbd3.S antibody, mbd3 antibody, MBD3 antibody, Mbd3 antibody
    Background
    DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
    Molecular Weight
    32844
    Gene ID
    53615
    NCBI Accession
    NP_001268382, NP_001268383
    UniProt
    O95983
    Pathways
    Chromatin Binding
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