Histone Cluster 3, H3 (HIST3H3) (AA 1-30), (meLys9) antibody

Details for Product No. ABIN387902
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Antigen
Synonyms H3.4, H3/g, H3FT, H3t, CG33848, Dmel\CG33848, H3.2-221, Hist1, H3/i, H3FI, h3, Hist1h3f, hist2h3c, T6J4.12, T6J4_12
Epitope
AA 1-30, meLys9
(50), (42), (37), (30), (29), (24), (24), (23), (20), (19), (19), (18), (17), (17), (17), (15), (15), (11), (10), (10), (9), (9), (9), (8), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(660), (393), (391), (179), (169), (12), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(600), (76), (5), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(29), (21), (20), (18), (17), (17), (17), (17), (17), (17), (17), (11), (11), (11), (2)
Application
Dot Blot (DB)
(486), (206), (184), (172), (169), (81), (61), (59), (41), (38), (27), (2), (1), (1)
Pubmed 3 references available
Quantity 400 μL
Options
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Catalog No. ABIN387902
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Immunogen This Histone H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from human Histone H3.
Clone RB1169
Isotype Ig
Specificity This Histone H3-MeK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide methylated on K9(2 methyl groups) between 1~30 amino acids from aa 6~12 of human Histone 3.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Alternative Name Histone H3
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene for histone H3-MeK9 is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Synonyms: Histone H3
Molecular Weight 15273 DA
Gene ID 3021
UniProt P84243
Research Area Protein Modifications, Cancer, Chromatin, Signaling, Cell Structure
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Histone Cluster 3, H3 (HIST3H3) (AA 1-30), (meLys9) antibody Dot blot analysis of anti-Methyl-K-H3-K9(2Me)-4MAPS Pab (ABIN387902) on nitrocellulose membrane. 50 ng of Methyl-peptide or Non Methyl-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
Background publications Albig, Kioschis, Poustka et al.: "Human histone gene organization: nonregular arrangement within a large cluster." in: Genomics, Vol. 40, Issue 2, pp. 314-22, 1997 (PubMed).

Albig, Doenecke: "The human histone gene cluster at the D6S105 locus." in: Human genetics, Vol. 101, Issue 3, pp. 284-94, 1998 (PubMed).

Marzluff, Gongidi, Woods et al.: "The human and mouse replication-dependent histone genes." in: Genomics, Vol. 80, Issue 5, pp. 487-98, 2002 (PubMed).

Validation Images
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