Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibody

Antigen: Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387908

Additional Information

Alternative name: UBE1C

Gene ID: 9039

UniProt: Q8TBC4

Immunogen: This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~67 amino acids from the N-terminal region of human UBE1C.

Isotype: Ig

Description: Other names: Ubiquitin-activating enzyme E1C

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~67 amino acids from the N-terminal region of human UBE1C.

Molecular Weight: 51719 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot~~ 1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UBE1C antibody (N-term) (ABIN387908) in mouse heart tissue lysates (35ug/lane). UBE1C (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

UBE1C Antibody (L45) (ABIN387908) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).

Publications

Product: Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).

MacKay, Mack, Brophy et al.: "The Bath Ankylosing Spondylitis Radiology Index (BASRI): a new, validated approach to disease assessment." in: Arthritis and rheumatism, Vol. 41, Issue 12, pp. 2263-70, 1999 (PubMed).